An automated method to analyze root filling voids and gaps using confocal microscopy images.

This study evaluated the feasibility of an automated method to delimit the required area to quantitatively analyze root filling voids and gaps from cross-sectional confocal laser scanning microscopy (CLSM) images. Root canals of maxillary canines were prepared with rotary instruments and filled by lateral compaction technique using gutta-percha and AH Plus sealer. The roots were stored (100% humidity, 37 °C) for a period of 24 h and then transversally sectioned to obtain 2-mm-thick slices from the apical and middle thirds. The areas corresponding to filling materials, gaps, and voids were manually delimited or automatically demarked by ImageJ software after converting the images to the RGB color system. Based on manual and automatic delimitations, the percentages of voids and gaps were calculated. Data of voids and gaps between middle and apical thirds were individually compared by paired t-test. Pearson`s correlation test was used to assess the correlation of data between the methods. Irrespective of the method of area delimitation, no difference was observed between the root thirds for both voids and gaps, while the p-values calculated for each method were similar. Almost perfect correlations between the methods were observed for both outcomes. The proposed method to automatically delimit the areas corresponding to filling material, voids, and gaps appears to be a valid method to facilitate the quantitative analysis of defects in root canal fillings using topographic CSLM images.

Polymicrobial Leakage and Retention of MTA and Portland Cement in a Model of Apexification

Abstract Objective: To evaluate the sealing capacity and retention of apical barriers made with mineral trioxide aggregate (MTA) and Portland cement (PC). Material and Methods: Fifty-six bovine incisors were sectioned 8 mm above and 12 mm below the cement-enamel junction. The root canal was enlarged with a diamond drill to create a standard 2.5 mm diameter opening. Apical sheets of 5 mm thickness were placed using white MTA-Angelus or white PC. Fifteen samples of each material were exposed to human saliva in a dual chamber apparatus and casting was evaluated at 30 days. Samples without apical barriers and fully sealed samples were used as positive and negative controls (n = 3), respectively. Data were analyzed by Fisher's exact test (p<0.05) after 3 periods: 1 to 10 days (P1); days 11 to 20 (P2); and days 21 to 30 (P3). Then, three 1 mm thick sections were obtained at the apical level of other root samples of each material (n = 10) and the push-out test was performed Results: The leakage rates in P1, P2 and P3 were 60%, 73.3% and 100% for the MTA; and 73.3%, 86.7% and 100% for CP, with no significant difference between materials, regardless of the period analyzed. There were no significant differences between the bond strengths for both cements (p>0.05) Conclusion: Mineral trioxide aggregate and Portland cement apical barriers presented similar sealing ability and bond strength values.

Alternative techniques to remove fractured instrument fragments from the apical third of root canals: report of two cases.

This paper describes two cases of instrument fragment removal from the apical thirds of root canals using a customized extractor and a modified needle technique, respectively. In case 1, a customized extractor was manufactured to remove a bur fragment located in the apical root canal of a maxillary central incisor. The use of this extractor enabled successful and conservative removal of the instrument fragment. In case 2, a modified injection needle was used as a trepan to gain access around an instrument fragment located in the curved apical portion of the mesiobuccal canal of a mandibular molar. A segment of steel wire was inserted into the needle lumen to engage the metallic fragment, enabling its removal with counter-clockwise rotation and a simultaneous pull-out motion. Alternative and creative methods are useful for the management of intracanal metallic fragments during root canal treatment.

Alternative Techniques to Remove Fractured Instrument Fragments from the Apical Third of Root Canals: Report of Two Cases

This paper describes two cases of instrument fragment removal from the apical thirds of root canals using a customized extractor and a modified needle technique, respectively. In case 1, a customized extractor was manufactured to remove a bur fragment located in the apical root canal of a maxillary central incisor. The use of this extractor enabled successful and conservative removal of the instrument fragment. In case 2, a modified injection needle was used as a trepan to gain access around an instrument fragment located in the curved apical portion of the mesiobuccal canal of a mandibular molar. A segment of steel wire was inserted into the needle lumen to engage the metallic fragment, enabling its removal with counter-clockwise rotation and a simultaneous pull-out motion. Alternative and creative methods are useful for the management of intracanal metallic fragments during root canal treatment.

Este relato descreve dois casos de remoção de fragmentos de instrumentos de terços apicais de canais radiculares utilizando um extrator personalizado e técnica da agulha modificada, respectivamente. No Caso 1, um extrator foi fabricado para remover fragmento de broca localizado no terço apical do canal radicular de um incisivo central superior. O uso deste extrator permitiu a remoção bem sucedida e conservadora do fragmento de instrumento. No Caso 2, uma agulha para anestesia modificada foi utilizada como um trépano para ganhar acesso ao redor de um fragmento de instrumento localizado na porção apical curva do canal mésio-vestibular de um molar inferior. Um segmento de fio de aço foi inserido no lúmen da agulha para envolver o fragmento metálico, permitindo sua remoção com rotação no sentido anti-horário e simultâneo movimento de retração. Métodos alternativos e criativos são úteis para a remoção intracanal de fragmentos metálicos durante o tratamento endodôntico.

Obturation Over an S1 ProTaper Instrument Fragment in a Mandibular Molar with Three Years of Follow-up

This case report describes root canal filling performed over a large S1 ProTaper file fragment in a second mandibular molar with irreversible pulpitis. An S1 ProTaper file was fractured during the instrumentation of the mesiobuccal canal. Approximately 10 mm of file fragment remained in the apical and middle thirds of the canal. The obturation was performed over this fragment using thermomechanically compacted gutta-percha and sealer. Radiographic findings and the absence of clinical signs and symptoms at 3-year follow up indicated successful treatment. Cone-beam computed tomography images revealed absence of periapical lesion and details of intracanal file fragment related to root fillings and apex morphology. In this case, the presence of a large intracanal fractured instrument did not have a negative impact on the endodontic prognosis during the follow up evaluation period.

Este relato de caso descreve a obturação do canal radicular realizada sobre um grande fragmento da lima ProTaper S1 em um segundo molar inferior com pulpite irreversível. Uma lima ProTaper S1 fraturou durante a instrumentação do canal mésio-vestibular. Aproximadamente 10 mm de remanescente do fragmento da lima permaneceu nos terços apical e médio do canal. A obturação foi realizada sobre este fragmento usando guta-percha compactada termomecanicamente e cimento endodôntico. Achados radiográficos e ausência de sinais e sintomas clínicos após 3 anos de acompanhamento indicaram o sucesso do tratamento. Imagens de tomografia computadorizada de feixes cônicos revelaram a ausência de lesão periapical e detalhes do fragmento da lima intracanal relacionados à obturação do canal radicular e à morfologia do ápice. Neste caso, a presença de grande instrumento fraturado intracanal não teve impacto negativo no prognóstico endodôntico durante o período de acompanhamento.

Apical transportation associated with ProTaper Universal F1, F2 and F3 instruments in curved canals prepared by undergraduate students

Objective: This study evaluated apical transportation associated with ProTaper® Universal Fl, F2 and F3 rotary files in curved canais prepared by undergraduate students. Material and Methods: Twenty mesial roots of mandibular molars with curvatures ranging between 25° and 35° were selected. Mesiobuccal canals were instrumented by twenty students with the ProTaper® system (Dentsply-Maillefer, Ballaigues, Switzerland) according to the manufacturer's instructions. Pre-fiaring was performed with Sl and SX files. A #15 K-file was inserted into the root canal up to the working length (WL), and an initial digital radiograph was taken in a buccolingual direction (baseline). Afterwards, the S1, S2, F1, F2, and F3 files were employed up to the WL. Other radiographies were taken in the same orientation of the baseline after the use of the Fl, F2, and F3 files, with each file inserted into the root canal. The radiographic images were overlapped, and the Image J software was used to measure the distance between the rotary files' ends and the #15 K-file's end, characterizing the apical transportation. Data were analyzed by Repeated Measure ANOVA and by the SNK post hoc test (P<0.05). Results: It was verified that file size affected apical transportation significantly (P<0.001). The F3 file showed higher apical transportation than Fl and F2, while between these last files there was no difference. Conclusion: The undergraduate students produced lower apical transportation in curved canals when they did not use the F3 rotary file. .

Apical transportation associated with ProTaper Universal F1, F2 and F3 instruments in curved canals prepared by undergraduate students.

OBJECTIVE: This study evaluated apical transportation associated with ProTaper Universal Fl, F2 and F3 rotary files in curved canais prepared by undergraduate students. MATERIAL AND METHODS: Twenty mesial roots of mandibular molars with curvatures ranging between 25° and 35° were selected. Mesiobuccal canals were instrumented by twenty students with the ProTaper system (Dentsply-Maillefer, Ballaigues, Switzerland) according to the manufacturer's instructions. Pre-fiaring was performed with Sl and SX files. A #15 K-file was inserted into the root canal up to the working length (WL), and an initial digital radiograph was taken in a buccolingual direction (baseline). Afterwards, the S1, S2, F1, F2, and F3 files were employed up to the WL. Other radiographies were taken in the same orientation of the baseline after the use of the Fl, F2, and F3 files, with each file inserted into the root canal. The radiographic images were overlapped, and the Image J software was used to measure the distance between the rotary files' ends and the #15 K-file's end, characterizing the apical transportation. Data were analyzed by Repeated Measure ANOVA and by the SNK post hoc test (P<0.05). RESULTS: It was verified that file size affected apical transportation significantly (P<0.001). The F3 file showed higher apical transportation than Fl and F2, while between these last files there was no difference. CONCLUSION: The undergraduate students produced lower apical transportation in curved canals when they did not use the F3 rotary file.

Obturation over an S1 ProTaper instrument fragment in a mandibular molar with three years of follow-up.

This case report describes root canal filling performed over a large S1 ProTaper file fragment in a second mandibular molar with irreversible pulpitis. An S1 ProTaper file was fractured during the instrumentation of the mesiobuccal canal. Approximately 10 mm of file fragment remained in the apical and middle thirds of the canal. The obturation was performed over this fragment using thermomechanically compacted gutta-percha and sealer. Radiographic findings and the absence of clinical signs and symptoms at 3-year follow up indicated successful treatment. Cone-beam computed tomography images revealed absence of periapical lesion and details of intracanal file fragment related to root fillings and apex morphology. In this case, the presence of a large intracanal fractured instrument did not have a negative impact on the endodontic prognosis during the follow up evaluation period.

Midbuccal Canals of Maxillary Molars Evaluated by Cone-Beam Computed Tomography: Endodontic Management of Two Cases

Maxillary molars present variable root canal and root morphologies. This report describes the endodontic management of two cases of midbuccal canals found in maxillary molars. Midbuccal canals were present in a maxillary first molar with a single buccal root (Case 1), and in a maxillary second molar with three buccal roots (Case 2). An assessment of the internal configuration of these teeth was performed using cone-beam computed tomography (CBCT). Magnification with a dental operating microscope, surgical loupes, and the use of an endodontic explorer enabled the identification of the midbuccal canal orifices. The root canals in both cases were chemomechanically prepared and filled. Postobturation radiographic images revealed four (one midbuccal, two mesiobuccal and one palatal) and five (one midbuccal, two mesiobuccal, one distobuccal and one palatal) root canals, which were filled in Cases 1 and 2, respectively. Complex canal configurations of maxillary molars including the presence of midbuccal canals were presented. CBCT was a valuable tool in this diagnosis, as it provided a precise description of these unusual anatomical variations.

Molares superiores apresentam morfologias radiculares e canais radiculares variáveis. Este relato de caso descreve o manejo endodôntico de dois casos de canais mésio-centrais (MC) encontrados em molares superiores. Canais MC estavam presente em um primeiro molar superior com uma única raiz vestibular (Caso 1), e em um segundo molar com três raízes vestibulares (Caso 2). Uma avaliação da configuração interna destes dentes foi realizada usando tomografia computadorizada de feixe cônico (TCFC). Ampliação com microscópio operatório odontológico, lupas cirúrgicas e o uso de um explorador endodôntico possibilitaram a identificação dos orifícios dos canais MC. Os canais radiculares dos dois casos foram preparados e obturados. Imagens radiográficas pós-obturação revelaram quatro (um MC, dois mésio-vestibulares e um palatino) e cinco (um MC, dois mésio-vestibulares, um disto-palatino e um palatino) canais radiculares, que foram obturados nos Casos 1 e 2, respectivamente. Configurações complexas de canais em molares superiores, incluindo a presença de canais MC foram apresentadas. TCFC foi uma ferramenta útil no diagnóstico e propiciou uma descrição precisa destas variações anatômicas incomuns.

Biocompatibilidade e capacidade de selamento do agregado de trióxido mineral em perfurações radiculares / Biocompatibility and sealing ability of mineral trioxide aggregate in root perforations

As perfurações radiculares rompem a barreira biológica entre o endodonto e o espaço periodontal, podendo, em razão da sua extensão e localização, comprometerem, irreversivelmente, a longevidade do elemento dentário. Do ponto de vista terapêutico, o agregado de trióxido mineral é o material selador mais recomendado. O agregado de trióxido mineral é apresentado sob a forma de um pó branco ou cinza, contendo partículas hidrofílicas, cujos componentes principais são silicato tricálcico, aluminato tricálcico, óxido tricálcico, óxido silicato e o óxido de bismuto, responsável pela radiopacidade, além dos elementos cromo e chumbo. Quando incorporado à água destilada, resulta em um gel coloidal que é aplicado diretamente no sítio da perfuração. Seu tempo de presa final é de aproximadamente 3 horas. Esse agregado apresenta satisfatórias propriedades físicas e químicas, bem como compatibilidade com os tecidos perirradiculares, independentemente da sua cor. In vitro, o agregado de trióxido mineral apresenta capacidade de selamento superior a outros biomateriais. No entanto, a maioria das investigações clínicas envolve relatos isolados de casos, com poucas evidências longitudinais dos resultados alcançados.

The alterations provoked by pathologies or iatrogenesis favor the invasion of the periodontium, communicating pulp cavity with periradiculares tissues. Currently, for the treatments of those root perforations, the sealer material more suitable is the mineral trioxide aggregate, that was reported for the first time in 1993 and introduced commercially in 1998. Mineral trioxide aggregate is presented under the form of a powder white or gray that, once incorporate to the distilled water, has an easy manipulation resulting in a colloidal gel whose final time setting is about 3 hours. That aggregate reaches appropriate physical and chemical properties as well as biocompatibility with the periradiculares tissues. In the current review, the use of mineral trioxide aggregate was approached as sealer material of root perforations in both in vitro and in vivo studies. It was evidenced that mineral trioxide aggregate presents good sealing ability, superior the other materials of larger longevity in the dentistry. However, most of the clinical investigations involve isolated case reports, with few longitudinal evidences of the reached results.

Three independent mesial canals in a mandibular molar: four-year followup of a case using cone beam computed tomography.

Endodontic treatment of mandibular molars is challenging because of variable root canal morphology. The nonsurgical endodontic management of a mandibular first molar presenting an independent middle mesial canal is reported. After coronal access, additional clinical inspection of the mesial canals' orifices and their interconnecting groove using an endodontic explorer and 4.5× loupes enabled the identification of the middle mesial canal orifice. All root canals were chemomechanically prepared and filled. The tooth was asymptomatic and functional after 4 years of followup. Cone beam computed tomography (CBCT) images revealed normal periapical status and three-dimensional (3D) anatomical aspects of the root canal system.

Artefacts in Cone Beam CT Mimicking an Extrapalatal Canal of Root-Filled Maxillary Molar.

Despite the advantages of cone-beam computed tomography (CBCT), the images provided by this diagnostic tool can produce artifacts and compromise accurate diagnostic assessment. This paper describes an endodontic treatment of a maxillary molar where CBCT images suggested the presence of a nonexistent third root canal in the palatal root. An endodontic treatment was performed in a first maxillary molar with palatal canals, and the tooth was restored with a cast metal crown. The patient returned four years later presenting with a discomfort in chewing, which was reduced after occlusal adjustment. CBCT was prescribed to verify additional diagnostic information. Axial scans on coronal, middle, and apical palatal root sections showed images similar to a third root canal. However, sagittal scans demonstrated that these images were artifacts caused by root canal fillings. A careful interpretation of CBCT images in root-filled teeth must be done to avoid mistakes in treatment.

Midbuccal canals of maxillary molars evaluated by cone-beam computed tomography: endodontic management of two cases.

Maxillary molars present variable root canal and root morphologies. This report describes the endodontic management of two cases of midbuccal canals found in maxillary molars. Midbuccal canals were present in a maxillary first molar with a single buccal root (Case 1), and in a maxillary second molar with three buccal roots (Case 2). An assessment of the internal configuration of these teeth was performed using cone-beam computed tomography (CBCT). Magnification with a dental operating microscope, surgical loupes, and the use of an endodontic explorer enabled the identification of the midbuccal canal orifices. The root canals in both cases were chemomechanically prepared and filled. Postobturation radiographic images revealed four (one midbuccal, two mesiobuccal and one palatal) and five (one midbuccal, two mesiobuccal, one distobuccal and one palatal) root canals, which were filled in Cases 1 and 2, respectively. Complex canal configurations of maxillary molars including the presence of midbuccal canals were presented. CBCT was a valuable tool in this diagnosis, as it provided a precise description of these unusual anatomical variations.

Effect of pre-flaring and file size on the accuracy of two electronic apex locators.

OBJECTIVE: This ex vivo study evaluated the effect of pre-flaring and file size on the accuracy of the Root ZX and Novapex electronic apex locators (EALs). MATERIAL AND METHODS: The actual working length (WL) was set 1 mm short of the apical foramen in the palatal root canals of 24 extracted maxillary molars. The teeth were embedded in an alginate mold, and two examiners performed the electronic measurements using #10, #15, and #20 K-files. The files were inserted into the root canals until the "0.0" or "APEX" signals were observed on the LED or display screens for the Novapex and Root ZX, respectively, retracting to the 1.0 mark. The measurements were repeated after the preflaring using the S1 and SX Pro-Taper instruments. Two measurements were performed for each condition and the means were used. Intra-class correlation coefficients (ICCs) were calculated to verify the intra- and inter-examiner agreement. The mean differences between the WL and electronic length values were analyzed by the three-way ANOVA test (p<0.05). RESULTS: ICCs were high (>0.8) and the results demonstrated a similar accuracy for both EALs (p>0.05). Statistically significant accurate measurements were verified in the pre-flared canals, except for the Novapex using a #20 K-file. CONCLUSIONS: The tested EALs showed acceptable accuracy, whereas the pre-flaring procedure revealed a more significant effect than the used file size.

Effect of pre-flaring and file size on the accuracy of two electronic apex locators

OBJECTIVE: This ex vivo study evaluated the effect of pre-flaring and file size on the accuracy of the Root ZX and Novapex electronic apex locators (EALs). MATERIAL AND METHODS: The actual working length (WL) was set 1 mm short of the apical foramen in the palatal root canals of 24 extracted maxillary molars. The teeth were embedded in an alginate mold, and two examiners performed the electronic measurements using #10, #15, and #20 K-files. The files were inserted into the root canals until the "0.0" or "APEX" signals were observed on the LED or display screens for the Novapex and Root ZX, respectively, retracting to the 1.0 mark. The measurements were repeated after the preflaring using the S1 and SX Pro-Taper instruments. Two measurements were performed for each condition and the means were used. Intra-class correlation coefficients (ICCs) were calculated to verify the intra- and inter-examiner agreement. The mean differences between the WL and electronic length values were analyzed by the three-way ANOVA test (p<0.05). RESULTS: ICCs were high (>0.8) and the results demonstrated a similar accuracy for both EALs (p>0.05). Statistically significant accurate measurements were verified in the pre-flared canals, except for the Novapex using a #20 K-file. CONCLUSIONS: The tested EALs showed acceptable accuracy, whereas the pre-flaring procedure revealed a more significant effect than the used file size.

Endodontic management of a long-standing horizontal mid-root fracture: case report in a young patient.

The purpose of this case report was to present the successful endodontic management of a long-standing horizontal mid-root fracture in a permanent maxillary central incisor. A 12-year-old boy with a history of traumatic injury sustained 15 months previously to the maxillary central incisor presented for treatment. Clinical examination revealed physiological mobility, pulp chamber exposed to the oral environment, and the buccal mucosa with a sinus tract in the area of the traumatized tooth. A radiolucent lesion at the fracture line and apical fragment with obliteration of the root canal space were observed radiographically. Conservative root canal treatment of the coronal segment was performed using calcium hydroxide as an intracanal dressing. The calcium hydroxide was replaced every 8 weeks for 10 months. A hard tissue barrier was observed after this period, and the coronal fragment of the root canal was filled. After 4 years, the tooth was asymptomatic and functional and no periapical lesion was observed.

Orthograde retreatment failure with extruded MTA apical plug in a large periradicular lesion followed by surgical intervention: case report.

Absence of periapical healing after orthograde retreatment using an apical plug with mineral trioxide aggregate (MTA) can require surgical intervention. A patient with a root-filled maxillary central incisor with chronic apical periodontitis and sinus tract was referred for endodontic retreatment. Excessive apical enlargement was verified, indicating an MTA apical plug placement; however, an unintentional extrusion of MTA occurred during this step. The root canal was filled with gutta-percha and sealer, and periodic recalls were scheduled. The sinus tract was observed after six months and the lesion remained unaltered, although the extruded MTA had resorbed; therefore, an apicoectomy and retrograde root-end filling with MTA were performed. Absence of sinus tract, normal clinical aspect, and complete repair of the lesion were observed at the two-year follow-up.

Estudo bibliométrico de artigos brasileiros publicados em periódicos internacionais de Endodontia: período 2008-2010 / Bibliometric study of Brazilian manuscripts published in international journals of endodontics: 2008-2010

Objetivo: Investigou-se a representação brasileira nas publicações dos periódicos Journal of Endodontics (J Endod) e International Endodontic Journal (Int Endod J), bem como o perfil dos estudos brasileiros publicados nos dois periódicos. Materiais e Métodos: Todos os artigos publicados entre janeirode 2008 a dezembro de 2010 nos periódicos J Endod e Int Endod J foram acessados por meio do portal deperiódicos da CAPES. Dois pesquisadores avaliaram os artigos quanto ao delineamento, ao tema abordado e ao país de origem. Os estudos brasileiros foram ainda caracterizados quanto ao Estado e instituição de origem dos autores. Foi realizada uma análise descritiva dos dados empregando-se o programa SPSS 17.0. Resultados: Foram publicados, neste período, 1381 artigos (988 no J Endod; 393 no Int Endod J). O Brasilocupou o 2º lugar na produção de artigos científicos, apresentando 232 publicações (16,7%). A maioria dosestudos que geraram essas publicações foram desenvolvidos a partir de experimentos laboratoriais (82,7%)que abordaram principalmente os temas relacionados aos materiais e instrumentos utilizados na práticaendodôntica (55,9%) e à microbiologia endodôntica (23,8%). A maior parte deles eram provenientes de universidades públicas do Estado de São Paulo. Conclusão: O número de artigos brasileiros publicados nos últimos três anos nos periódicos J Endod e Int Endod J foi expressivo, alcançando o segundo lugar mundial.

Nonsurgical endodontic management using MTA for perforative defect of internal root resorption: report of a long term follow-up.

Internal root resorption is an uncommon lesion following a dental injury. The use of mineral trioxide aggregate (MTA) is a conservative approach to repair lesions with periodontal communication. This case report presents a long-term follow-up of a nonsurgical endodontic management using MTA for perforative defect of internal root resorption. During the endodontic treatment, the granulation tissue was removed and the root canal prepared. Calcium hydroxide was placed as a temporary dressing for 30 days. After this period, the root canal space and the perforation defect were filled with MTA. The clinical findings and periapical radiographs indicated success of treatment until 2 years of follow-up. However, the radiograph after 8 years showed an extensive radiolucent area in the middle third of the root with separation of the apical and coronal root segments. These findings were observed more accurately by using cone-beam computerized tomography.

Effect of the simulated periodontal ligament on cast post-and-core removal using an ultrasonic device.

OBJECTIVE: The aim of this study was to evaluate the effect of simulated periodontal ligament (SPDL) on custom cast dowel and core removal by ultrasonic vibration. MATERIAL AND METHODS: Thirty-two human maxillary canines were included in resin cylinders with or without SPDL made from polyether impression material. In order to allow tensile testing, the roots included in resin cylinders with SPDL were fixed to cylinders with two stainless steel wires. Post-holes were prepared by standardizing the length at 8 mm and root canal impressions were made with self-cured resin acrylic. Cast dowel and core sets were fabricated and luted with Panavia F resin cement. Half of the samples were submitted to ultrasonic vibration before the tensile test. Data were analyzed statistically by two-way ANOVA and Tukey's post-hoc tests (p<0.05). RESULTS: The ultrasonic vibration reduced the tensile strength of the samples directly included in resin cylinders. There was no difference between the values, whether or not ultrasonic vibration was used, when the PDL was simulated. However, the presence of SPDL affected the tensile strength values even when no ultrasonic vibration was applied. CONCLUSION: Simulation of PDL has an effect on both ultrasonic vibration and tensile testing.